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Having done the 50k L version: foam control alone eats the margin they are projecting.
We got a term sheet last year that explicitly cut the CMC budget to make the burn look better. Declined.
This is the third time this month someone has posted a "cheap X" build where the cost excludes the thing that actually costs money. Where is the temperature controller in that BOM?
Off-target analysis with GUIDE-seq only tells you about the sites you could capture. I would want long-read whole-genome on at least a few animals before "no detectable" goes in a title.
Which is the argument for making it a procurement requirement rather than a law — funders can move faster than legislatures.
Started my lab entirely on auction equipment. The trick is buying from a closing pharma site rather than a university surplus sale — pharma gear comes with service records.
We got a term sheet last year that explicitly cut the CMC budget to make the burn look better. Declined.
Open datasets like this are the single highest-leverage thing a lab can publish. Papers age, data compounds.
The border question has no general answer. It is per-country, per-organism, and sometimes per-customs-officer. Talk to the receiving institution first, always.
Rewriting firmware in Rust is fun but the actual reliability win here was the watchdog, which you could have added in C.
This matches my experience. Also: never buy a -80 freezer sight unseen.
Strongly agree. Our incident log is 90% solvent handling and 10% sharps. Zero biological.
Open datasets like this are the single highest-leverage thing a lab can publish. Papers age, data compounds.
We run exactly this curriculum. Two years, zero incidents, several very good student questions we could not answer.
The ramp rate claim is doing a lot of work here — is that block temperature or sample temperature? Those diverge badly above 2 °C/s with thin-wall tubes.
Add: get the material transfer agreement signed before shipping, not after. Customs asks for paperwork, not for vibes.
Strongly agree. Our incident log is 90% solvent handling and 10% sharps. Zero biological.
I would like to see the word "intelligence" retired from this entire subfield until someone proposes a falsifiable behavioural test.
Having done the 50k L version: foam control alone eats the margin they are projecting.
Fair. The preprint does have ONT data in supplementary S4, though the coverage is thin.
This is the most honest write-up of seed terms I have read. The part about pro-rata rights is the part founders always skip.
Started my lab entirely on auction equipment. The trick is buying from a closing pharma site rather than a university surplus sale — pharma gear comes with service records.
Sample temperature, measured with a thermocouple in a dummy tube of mineral oil. Plot is in the repo under docs/thermal.
Screening baseline is good but voluntary. Voluntary regimes work until one provider decides growth matters more.
Because metadata is cheap to advocate for and replication is not.
Biosafety take: none of this is a problem at BSL-1 with non-pathogenic chassis. The failure mode in community labs is almost never the organism, it is the chemical waste.
True, and said so in the README. The Rust part was mostly so contributors stop segfaulting the pH loop.
Which is the argument for making it a procurement requirement rather than a law — funders can move faster than legislatures.
The teardown is beautiful. Note the flow cell is still proprietary, so "open" has a ceiling here.
Off-target analysis with GUIDE-seq only tells you about the sites you could capture. I would want long-read whole-genome on at least a few animals before "no detectable" goes in a title.