Strongly agree. Our incident log is 90% solvent handling and 10% sharps. Zero biological.

Biosafety take: none of this is a problem at BSL-1 with non-pathogenic chassis. The failure mode in community labs is almost never the organism, it is the chemical waste.

Off-target analysis with GUIDE-seq only tells you about the sites you could capture. I would want long-read whole-genome on at least a few animals before "no detectable" goes in a title.

It is in there — line 14, the $22 board. The expensive part historically was the Peltier driver and that is what got cheap.

Fair. The preprint does have ONT data in supplementary S4, though the coverage is thin.

This is the third time this month someone has posted a "cheap X" build where the cost excludes the thing that actually costs money. Where is the temperature controller in that BOM?