LIVE 32 submissions / 186 comments / 1548 upvotes▲72 Show BN: A $180 open-source thermocycler that hits 3 °C/s ramp rates▲63 Base editor corrects a point mutation in adult mouse liver with no detectable off-targets▲53 Ask BN: How do you validate a community lab BSL-1 setup without an institution behind you?▲49 A cell-free protein synthesis kit you can freeze-dry at home▲47 The reproducibility crisis is a metadata crisis▲44 Show BN: I sequenced my sourdough starter and built a strain tracker for it// The revolution will not be peer reviewed.LIVE 32 submissions / 186 comments / 1548 upvotes▲72 Show BN: A $180 open-source thermocycler that hits 3 °C/s ramp rates▲63 Base editor corrects a point mutation in adult mouse liver with no detectable off-targets▲53 Ask BN: How do you validate a community lab BSL-1 setup without an institution behind you?▲49 A cell-free protein synthesis kit you can freeze-dry at home▲47 The reproducibility crisis is a metadata crisis▲44 Show BN: I sequenced my sourdough starter and built a strain tracker for it// The revolution will not be peer reviewed.

API

JSON over the same data the pages render. Read endpoints are open; writes need a session.

GET /api/stories Ranked front page. ?page, ?limit, ?topic, ?sort=top|new|best|ask|show
GET /api/item/:id One story or comment, with its comment tree when it is a story.
GET /api/user/:id Public profile: karma, about, counts.
GET /api/search?q= Full listing search.
GET /api/topics Channels and their submission counts.
POST /api/vote { id, dir: "up"|"down" } — session cookie + X-CSRF-Token required.
POST /api/submit { title, url?, text?, topic? } — returns the new item.
POST /api/comment { parent, text } — returns the new comment.
GET /rss RSS 2.0 of the current front page.

Rate limit: 60 requests/minute per IP on reads, tighter on writes. Be kind — it is one small server in a shared incubator.