BIOPUNK//NEWS
  1. Directed evolution in a droplet: 10^7 variants per afternoon (biorxiv.org) Synthetic Biology 16 points | plasmid_mule | | 6 comments

Log in to join the thread.

6 comments in thread
[–] wetware_ann | 5 points | link

Anyone else notice that the reproducibility argument always ends with "we need better metadata" and never with "we need to fund replication"?

[–] micron52 | 3 points | link

The cost curve here is real but the pilot scale is 500 L. Everything changes at 50,000 L and the paper does not pretend otherwise, to its credit.

[–] blot16 | 4 points | link

Having done the 50k L version: foam control alone eats the margin they are projecting.

[–] plasmid_mule op | 3 points | link

Because metadata is cheap to advocate for and replication is not.

[–] buffer24 | 3 points | link

Biosafety take: none of this is a problem at BSL-1 with non-pathogenic chassis. The failure mode in community labs is almost never the organism, it is the chemical waste.

[–] plasmid_mule op | 3 points | link

Strongly agree. Our incident log is 90% solvent handling and 10% sharps. Zero biological.