LIVE 32 submissions / 186 comments / 1548 upvotes▲72 Show BN: A $180 open-source thermocycler that hits 3 °C/s ramp rates▲63 Base editor corrects a point mutation in adult mouse liver with no detectable off-targets▲53 Ask BN: How do you validate a community lab BSL-1 setup without an institution behind you?▲49 A cell-free protein synthesis kit you can freeze-dry at home▲47 The reproducibility crisis is a metadata crisis▲44 Show BN: I sequenced my sourdough starter and built a strain tracker for it// The revolution will not be peer reviewed.LIVE 32 submissions / 186 comments / 1548 upvotes▲72 Show BN: A $180 open-source thermocycler that hits 3 °C/s ramp rates▲63 Base editor corrects a point mutation in adult mouse liver with no detectable off-targets▲53 Ask BN: How do you validate a community lab BSL-1 setup without an institution behind you?▲49 A cell-free protein synthesis kit you can freeze-dry at home▲47 The reproducibility crisis is a metadata crisis▲44 Show BN: I sequenced my sourdough starter and built a strain tracker for it// The revolution will not be peer reviewed.
We ran this protocol in our community lab last month. Two of five transformations failed until we dropped the heat shock to 42 s. Worth adding a note.