Sample temperature, measured with a thermocouple in a dummy tube of mineral oil. Plot is in the repo under docs/thermal.

Trehalose stabilisation is old but the field-deployment data is what makes this worth reading. 40 °C for six weeks is a real number.

The teardown is beautiful. Note the flow cell is still proprietary, so "open" has a ceiling here.

We run exactly this curriculum. Two years, zero incidents, several very good student questions we could not answer.

Teaching teenagers: use non-pathogenic yeast, keep everything on plates, and let them design the experiment. The safety risk is boredom-driven improvisation, not the enzyme.

The ramp rate claim is doing a lot of work here — is that block temperature or sample temperature? Those diverge badly above 2 °C/s with thin-wall tubes.