LIVE 32 submissions / 186 comments / 1548 upvotes▲72 Show BN: A $180 open-source thermocycler that hits 3 °C/s ramp rates▲63 Base editor corrects a point mutation in adult mouse liver with no detectable off-targets▲53 Ask BN: How do you validate a community lab BSL-1 setup without an institution behind you?▲49 A cell-free protein synthesis kit you can freeze-dry at home▲47 The reproducibility crisis is a metadata crisis▲44 Show BN: I sequenced my sourdough starter and built a strain tracker for it// The revolution will not be peer reviewed.LIVE 32 submissions / 186 comments / 1548 upvotes▲72 Show BN: A $180 open-source thermocycler that hits 3 °C/s ramp rates▲63 Base editor corrects a point mutation in adult mouse liver with no detectable off-targets▲53 Ask BN: How do you validate a community lab BSL-1 setup without an institution behind you?▲49 A cell-free protein synthesis kit you can freeze-dry at home▲47 The reproducibility crisis is a metadata crisis▲44 Show BN: I sequenced my sourdough starter and built a strain tracker for it// The revolution will not be peer reviewed.

Comments by lysate44

[–] ▲ lysate44 | 4 points | 11 hours ago link |on Screening DNA synthesis orders: the new baseline every provider should meet

The teardown is beautiful. Note the flow cell is still proprietary, so "open" has a ceiling here.

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[–] ▲ lysate44 | 5 points | 1 day ago link |on A ten-line protocol that doubled our transformation efficiency

The border question has no general answer. It is per-country, per-organism, and sometimes per-customs-officer. Talk to the receiving institution first, always.

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[–] ▲ lysate44 | 5 points | 1 day ago link |on Gene therapy pricing is a manufacturing problem, not a science problem

Every time gene therapy pricing comes up someone says "manufacturing" and then nobody funds manufacturing. It is the least glamorous line item in every deck I have seen.

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