LIVE 32 submissions / 186 comments / 1548 upvotes▲72 Show BN: A $180 open-source thermocycler that hits 3 °C/s ramp rates▲63 Base editor corrects a point mutation in adult mouse liver with no detectable off-targets▲53 Ask BN: How do you validate a community lab BSL-1 setup without an institution behind you?▲49 A cell-free protein synthesis kit you can freeze-dry at home▲47 The reproducibility crisis is a metadata crisis▲44 Show BN: I sequenced my sourdough starter and built a strain tracker for it// The revolution will not be peer reviewed.LIVE 32 submissions / 186 comments / 1548 upvotes▲72 Show BN: A $180 open-source thermocycler that hits 3 °C/s ramp rates▲63 Base editor corrects a point mutation in adult mouse liver with no detectable off-targets▲53 Ask BN: How do you validate a community lab BSL-1 setup without an institution behind you?▲49 A cell-free protein synthesis kit you can freeze-dry at home▲47 The reproducibility crisis is a metadata crisis▲44 Show BN: I sequenced my sourdough starter and built a strain tracker for it// The revolution will not be peer reviewed.

Comments by lysate30

[–] ▲ lysate30 | 4 points | 4 hours ago link |on The reproducibility crisis is a metadata crisis

Rewriting firmware in Rust is fun but the actual reliability win here was the watchdog, which you could have added in C.

[–] ▲ lysate30 | 4 points | 22 hours ago link |on The FDA draft guidance on decentralised manufacturing, annotated

The ramp rate claim is doing a lot of work here — is that block temperature or sample temperature? Those diverge badly above 2 °C/s with thin-wall tubes.