Comments by incubate21

Sample temperature, measured with a thermocouple in a dummy tube of mineral oil. Plot is in the repo under docs/thermal.

We ran this protocol in our community lab last month. Two of five transformations failed until we dropped the heat shock to 42 s. Worth adding a note.

Screening baseline is good but voluntary. Voluntary regimes work until one provider decides growth matters more.

Add: get the material transfer agreement signed before shipping, not after. Customs asks for paperwork, not for vibes.

The border question has no general answer. It is per-country, per-organism, and sometimes per-customs-officer. Talk to the receiving institution first, always.

Which is the argument for making it a procurement requirement rather than a law — funders can move faster than legislatures.