LIVE 32 submissions / 186 comments / 1548 upvotes▲72 Show BN: A $180 open-source thermocycler that hits 3 °C/s ramp rates▲63 Base editor corrects a point mutation in adult mouse liver with no detectable off-targets▲53 Ask BN: How do you validate a community lab BSL-1 setup without an institution behind you?▲49 A cell-free protein synthesis kit you can freeze-dry at home▲47 The reproducibility crisis is a metadata crisis▲44 Show BN: I sequenced my sourdough starter and built a strain tracker for it// The revolution will not be peer reviewed.LIVE 32 submissions / 186 comments / 1548 upvotes▲72 Show BN: A $180 open-source thermocycler that hits 3 °C/s ramp rates▲63 Base editor corrects a point mutation in adult mouse liver with no detectable off-targets▲53 Ask BN: How do you validate a community lab BSL-1 setup without an institution behind you?▲49 A cell-free protein synthesis kit you can freeze-dry at home▲47 The reproducibility crisis is a metadata crisis▲44 Show BN: I sequenced my sourdough starter and built a strain tracker for it// The revolution will not be peer reviewed.

Comments by ferment19

[–] ▲ ferment19 | 2 points | 4 hours ago link |on Precision fermentation hits $2/kg for a single-cell protein at pilot scale

It is in there — line 14, the $22 board. The expensive part historically was the Peltier driver and that is what got cheap.

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[–] ▲ ferment19 | 2 points | 17 hours ago link |on Precision fermentation hits $2/kg for a single-cell protein at pilot scale

Off-target analysis with GUIDE-seq only tells you about the sites you could capture. I would want long-read whole-genome on at least a few animals before "no detectable" goes in a title.

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[–] ▲ ferment19 | 3 points | 1 day ago link |on A cheap dye-free viability assay that works in undergrad teaching labs

The review actually proposes three. Section 4. They are weak, but they exist.

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